Authenticity Identification of Saccharum officinarum and Saccharum spontaneum Germplasm Materials

Sugarcane is an important sugar and energy crop in the world. Germplasm innovation a significant way to breed breakthrough sugarcane varieties. Modern varieties all contain blood relationship of Saccharum officinarum spontaneum. High results from S. resistance genes In order improve quality, breeders use spontaneum cross obtain hybrid offspring with high resistance. Therefore, authenticity progeny materials directly affects efficiency breeding. this study, tetra-primer amplification hindered mutation system (ARMS PCR) was used identify ten suspected eleven germplasm materials, then further validated by chromosome counting genome situ hybridization (GISH). Among be identified, three were real they 14NG124, 51NG103, Guan A. Nine identified fake these Yunge 2007-12-165, Guangxi 87-20, Yunnan 82-16, 2007-11, YNLC 16, Laos No. 2, 82-29, 2015-83, 2013-20. The ARMS PCR same as GISH results. had 80 chromosomes. Using GISH, nine proven authentic. Through number statistics, it found that Authentic selected enrich genetic background through reduce influence on breeding process misuse